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human recombinant fgf4  (MedChemExpress)


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    Structured Review

    MedChemExpress human recombinant fgf4
    Human Recombinant Fgf4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+fgf4/FGF-4%2C+Human/pm41212516-345-39-42
    Average 94 stars, based on 5 article reviews
    human recombinant fgf4 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development.
    Article Snippet: .. All TSLs were cultured on Matrigel-coated plates, in 30% TS medium (RPMI 1640 (GIBCO, 11875119), 20% FBS, 1% GlutaMax (GIBCO, 35050061), 1% penicillin-streptomycin (GIBCO, 15140163), 1% sodium pyruvate (GIBCO, 11360070)) and 70% MEF-conditioned TS medium supplemented with 25 ng/ml human recombinant FGF4 (MCE, HY-P7014) and 1 μg/ml heparin (STEMCELL, 7980). .. For longer culture, the medium was changed to serum-free TX medium (Garreta et al., 2021; Kim et al., 2020; Rossi et al., 2018): F12/DMEM (GIBCO, 11330057), 64 mg/L L-ascorbic acid-2-phosphate magnesium, 1% penicillin-streptomycin, 1% sodium pyruvate, and 2% InsulinTransferrin-Selenium-X (GIBCO, 51500056), supplemented with 25 ng/mL human recombinant FGF4, 1 μg/mL heparin, and 2 ng/mL mouse recombinant TGF-β1 (PeproTech, 100-21-10).

    Article Title: Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development
    Article Snippet: .. All TSLs were cultured on Matrigel-coated plates, in 30% TS medium (RPMI 1640 (GIBCO, 11875119), 20% FBS, 1% GlutaMax (GIBCO, 35050061), 1% penicillin-streptomycin (GIBCO, 15140163), 1% sodium pyruvate (GIBCO, 11360070)) and 70% MEF-conditioned TS medium supplemented with 25 ng/ml human recombinant FGF4 (MCE, HY-P7014) and 1 μg/ml heparin (STEMCELL, 7980). .. For longer culture, the medium was changed to serum-free TX medium (Garreta et al., 2021; Kim et al., 2020; Rossi et al., 2018): F12/DMEM (GIBCO, 11330057), 64 mg/L L-ascorbic acid-2-phosphate magnesium, 1% penicillin-streptomycin, 1% sodium pyruvate, and 2% Insulin-Transferrin-Selenium-X (GIBCO, 51500056), supplemented with 25 ng/mL human recombinant FGF4, 1 μg/mL heparin, and 2 ng/mL mouse recombinant TGF-β1 (PeproTech, 100-21-10).

    Recombinant:

    Article Title: Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development.
    Article Snippet: .. All TSLs were cultured on Matrigel-coated plates, in 30% TS medium (RPMI 1640 (GIBCO, 11875119), 20% FBS, 1% GlutaMax (GIBCO, 35050061), 1% penicillin-streptomycin (GIBCO, 15140163), 1% sodium pyruvate (GIBCO, 11360070)) and 70% MEF-conditioned TS medium supplemented with 25 ng/ml human recombinant FGF4 (MCE, HY-P7014) and 1 μg/ml heparin (STEMCELL, 7980). .. For longer culture, the medium was changed to serum-free TX medium (Garreta et al., 2021; Kim et al., 2020; Rossi et al., 2018): F12/DMEM (GIBCO, 11330057), 64 mg/L L-ascorbic acid-2-phosphate magnesium, 1% penicillin-streptomycin, 1% sodium pyruvate, and 2% InsulinTransferrin-Selenium-X (GIBCO, 51500056), supplemented with 25 ng/mL human recombinant FGF4, 1 μg/mL heparin, and 2 ng/mL mouse recombinant TGF-β1 (PeproTech, 100-21-10).

    Article Title: Capturing trophectoderm-like stem cells enables step-wisely remodeling of placental development
    Article Snippet: .. All TSLs were cultured on Matrigel-coated plates, in 30% TS medium (RPMI 1640 (GIBCO, 11875119), 20% FBS, 1% GlutaMax (GIBCO, 35050061), 1% penicillin-streptomycin (GIBCO, 15140163), 1% sodium pyruvate (GIBCO, 11360070)) and 70% MEF-conditioned TS medium supplemented with 25 ng/ml human recombinant FGF4 (MCE, HY-P7014) and 1 μg/ml heparin (STEMCELL, 7980). .. For longer culture, the medium was changed to serum-free TX medium (Garreta et al., 2021; Kim et al., 2020; Rossi et al., 2018): F12/DMEM (GIBCO, 11330057), 64 mg/L L-ascorbic acid-2-phosphate magnesium, 1% penicillin-streptomycin, 1% sodium pyruvate, and 2% Insulin-Transferrin-Selenium-X (GIBCO, 51500056), supplemented with 25 ng/mL human recombinant FGF4, 1 μg/mL heparin, and 2 ng/mL mouse recombinant TGF-β1 (PeproTech, 100-21-10).



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    Image Search Results


    The differentiation block in I-BET-resistant (I-BETR) diapause-like ES cells. ( A ) Alkaline phosphatase (AP) levels in control and I-BETR ES cells. Scale bar, 100 µm. ( B ) Expression levels of the pluripotency ( left panel) or differentiation-inducing ( right panel) genes in control, 2i-treated, or I-BETR ES cells. RNA expression levels were quantified by qPCR. Values represent normalized mean ± SD. n = 3. ( C ) Colony morphology and/or alkaline phosphatase (AP) levels in control and I-BETR ES cells following vehicle or FGF4 triggering. Scale bar, 100 µm. The bar graph represents quantification of relative percentage of pluripotent colonies in different groups. Values represent ± SD. n = 9. (n.s.) No significance, (****) P < 0.0001, one-way ANOVA with Dunnett's multiple comparisons test. ( D ) Expression levels of selected pluripotency ( left ) or differentiation-inducing ( right ) genes in control and I-BETR ES cells treated or not treated with FGF4. Error bars indicate SD. n = 3. (n.s.) No significance, (****) P < 0.0001, one-way ANOVA with Dunnett's multiple comparisons test. ( E ) Withdrawal of I-BET (I-BETW) restores the control ES cell-like pluripotency gene expression pattern in I-BETR ES cells. Values represent gene expression levels normalized to the mean of control samples based on TPM values observed by bulk mRNA RNA-seq analysis . ( F ) Generation of chimeras by I-BETR ES cells. The I-BETR ES cells were incubated in I-BET-free medium for 12–14 h and injected into the C57BL/6J blastocysts. White coat color indicates the chimerism.

    Journal: Genes & Development

    Article Title: Transcriptional derepression of negative regulators of MAP kinase supports maintenance of diapause ES cells in the pluripotent state

    doi: 10.1101/gad.353143.125

    Figure Lengend Snippet: The differentiation block in I-BET-resistant (I-BETR) diapause-like ES cells. ( A ) Alkaline phosphatase (AP) levels in control and I-BETR ES cells. Scale bar, 100 µm. ( B ) Expression levels of the pluripotency ( left panel) or differentiation-inducing ( right panel) genes in control, 2i-treated, or I-BETR ES cells. RNA expression levels were quantified by qPCR. Values represent normalized mean ± SD. n = 3. ( C ) Colony morphology and/or alkaline phosphatase (AP) levels in control and I-BETR ES cells following vehicle or FGF4 triggering. Scale bar, 100 µm. The bar graph represents quantification of relative percentage of pluripotent colonies in different groups. Values represent ± SD. n = 9. (n.s.) No significance, (****) P < 0.0001, one-way ANOVA with Dunnett's multiple comparisons test. ( D ) Expression levels of selected pluripotency ( left ) or differentiation-inducing ( right ) genes in control and I-BETR ES cells treated or not treated with FGF4. Error bars indicate SD. n = 3. (n.s.) No significance, (****) P < 0.0001, one-way ANOVA with Dunnett's multiple comparisons test. ( E ) Withdrawal of I-BET (I-BETW) restores the control ES cell-like pluripotency gene expression pattern in I-BETR ES cells. Values represent gene expression levels normalized to the mean of control samples based on TPM values observed by bulk mRNA RNA-seq analysis . ( F ) Generation of chimeras by I-BETR ES cells. The I-BETR ES cells were incubated in I-BET-free medium for 12–14 h and injected into the C57BL/6J blastocysts. White coat color indicates the chimerism.

    Article Snippet: For FGF4-driven ES cell differentiation, 10 ng/μL recombinant FGF4 (R&D Systems 235-F4) was added on day 0 together with 1 μg/μL heparin (Sigma-Aldrich H3149).

    Techniques: Blocking Assay, Control, Expressing, RNA Expression, Gene Expression, RNA Sequencing, Incubation, Injection